A/T-rich sequences act as quantitative enhancers of gene expression in transgenic tobacco and potato plants
Identifieur interne : 003034 ( Main/Exploration ); précédent : 003033; suivant : 003035A/T-rich sequences act as quantitative enhancers of gene expression in transgenic tobacco and potato plants
Auteurs : Jagdeep S. Sandhu [États-Unis] ; Carl I. Webster [Royaume-Uni] ; John C. Gray [Royaume-Uni]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 1998-07-01.
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Abstract
Abstract: The role of an A/T-rich positive regulatory region (P268, -444 to -177 from the translation start site) of the pea plastocyanin gene (PetE) promoter has been investigated in transgenic plants containing chimeric promoters fused to the β-glucuronidase (GUS) reporter gene. This region enhanced GUS expression in leaves of transgenic tobacco plants when fused in either orientation to a minimal pea PetE promoter (-176 to +4) and in roots when fused in either orientation upstream or downstream of a minimal cauliflower mosaic virus 35S promoter (-90 to +5). The region was also able to enhance GUS expression in microtubers of transgenic potato plants when placed in either orientation upstream of a minimal class I patatin promoter (-332 to +14). Dissection of P268 revealed that cis elements responsible for enhancing GUS expression from the minimal PetE promoter were distributed throughout P268. Multiple copies of a 31 bp A/T-rich sequence from within P268 and of a 26 bp random A/T sequence were able to enhance GUS expression from the minimal PetE promoter, indicating that A/T-rich sequences are able to act as quantitative, non-tissue-specific enhancer elements in higher plants. Abbreviations: CaMV, cauliflower mosaic virus; GUS, β-glucuronidase; HMG, high-mobility group; MAR, matrix-associated region; MU, methylumbelliferone; SAR, scaffold-associated region.
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DOI: 10.1023/A:1006051832213
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This region enhanced GUS expression in leaves of transgenic tobacco plants when fused in either orientation to a minimal pea PetE promoter (-176 to +4) and in roots when fused in either orientation upstream or downstream of a minimal cauliflower mosaic virus 35S promoter (-90 to +5). The region was also able to enhance GUS expression in microtubers of transgenic potato plants when placed in either orientation upstream of a minimal class I patatin promoter (-332 to +14). Dissection of P268 revealed that cis elements responsible for enhancing GUS expression from the minimal PetE promoter were distributed throughout P268. Multiple copies of a 31 bp A/T-rich sequence from within P268 and of a 26 bp random A/T sequence were able to enhance GUS expression from the minimal PetE promoter, indicating that A/T-rich sequences are able to act as quantitative, non-tissue-specific enhancer elements in higher plants. Abbreviations: CaMV, cauliflower mosaic virus; GUS, β-glucuronidase; HMG, high-mobility group; MAR, matrix-associated region; MU, methylumbelliferone; SAR, scaffold-associated region.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li><li>Illinois</li></region><settlement><li>Cambridge</li></settlement><orgName><li>Université de Cambridge</li></orgName></list><tree><country name="États-Unis"><region name="Illinois"><name sortKey="Sandhu, Jagdeep S" sort="Sandhu, Jagdeep S" uniqKey="Sandhu J" first="Jagdeep S." last="Sandhu">Jagdeep S. Sandhu</name></region></country><country name="Royaume-Uni"><noRegion><name sortKey="Webster, Carl I" sort="Webster, Carl I" uniqKey="Webster C" first="Carl I." last="Webster">Carl I. Webster</name></noRegion><name sortKey="Gray, John C" sort="Gray, John C" uniqKey="Gray J" first="John C." last="Gray">John C. 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